Antagonistic Activity of Aloe vera and Lactobacillus acidophilus Against Human Pathogens
D. Kannan, M. Manohar, Omran M. Abdulrahman, M. Madasamy Syed Wali Peeran
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Lactobacillus acidophilus is a member of the acidophilus group of intestinal Lactobacilli that have been extensively studied for their Probiotic activities that include pathogens inhibition, epithelial cell attachment and immunomodulation. Antimicrobial activity of the L.acidophilus was assessed by agar well assay techniques. L.acidophilus inoculated into broth and incubated at 37oC for 24 hours. Cell free extract was obtained by centrifugation and passed through the membrane filter and wells of 6 mm diameter made in each plate of Tryptone dextrose agar seeded with pathogenic organisms such as Escherichia coli, Salmonella sp, Shigella sp, Aspergillus niger, Candida albicans. Cell free extract of individual L.acidophilus culture was transferred at the rate of 50 ųl into each well. The antibiogram activity of L.acidophilus was compared with Aloe vera. So another well was filled with Aloe vera extract. The plates were kept in a refrigerator for 3 hours and allowed the diffusion of the sample into well followed by incubation at 37oC for 48 hours without inverting. Zone of inhibition formed around the well was measured. Zone inhibition of pathogenic organisms was tested.
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